MAGEA1 Antibody - middle region (OAAB16512)

• Product Number: OAAB16512
• Name: MAGEA1 Antibody - middle region (OAAB16512)
• Molecular Weight: 34342 Da
• Isotype: Ig
• NCBI Gene Id: 4100
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: CT1.1, MAGE1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: MAGEA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1 May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This MAGEA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-173 amino acids from the Central region of human MAGEA1.
• Additional Information: Cellular Location: Cytoplasm. Nucleus.
  Tissue Location: Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas
• Uniprot ID: P43355
• Protein Name: Melanoma-associated antigen 1
• Sample Type Confirmation:

  MAGEA1 is strongly supported by BioGPS gene expression data to be expressed in Hela

• Protein Accession #: NP_004979.3
• Gene Symbol: MAGEA1
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: Human lung carcinoma
  
  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MAGEA1 Antibody (Center) (Cat.# OAAB16512), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: Hela
  
  Western blot analysis of anti-MAGEA1 Antibody (Center) in Hela cell line lysates (35ug/lane). MAGEA1(arrow) was detected using the purified Pab (1:60dilution).

  MAGEA1 is strongly supported by BioGPS gene expression data to be expressed in Hela