LRP6 Antibody - C-terminal region (Phospho-T1546) (OAAB16507)

• Product Number: OAAB16507
• Name: LRP6 Antibody - C-terminal region (Phospho-T1546) (OAAB16507)
• Molecular Weight: 180429 Da
• Isotype: Ig
• NCBI Gene Id: 4040
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ADCAD2, STHAG7
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: LRP6 Antibody (C-term T1546) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity)
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This LRP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1531-1560 amino acids from the C-terminal region of human LRP6.
• Additional Information: Cellular Location: Membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Note=On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes
  Tissue Location: Widely coexpressed with LRP5 during embryogenesis and in adult tissues
• Uniprot ID: O75581
• Protein Name: Low-density lipoprotein receptor-related protein 6
• Sample Type Confirmation:

  LRP6 is supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_002327.2
• Gene Symbol: LRP6
• Predicted Species Reactivity: Human
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: 293
  
  Western blot analysis of LRP6 (arrow) using rabbit polyclonal LRP6 Antibody (C-term T1546) (Cat# OAAB16507). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LRP6 gene (Lane 2) (Origene Technologies).

  LRP6 is supported by BioGPS gene expression data to be expressed in HEK293

• Image 2: Human breast carcinoma
  
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with LRP6 Antibody (C-term T1546) (Cat.# OAAB16507), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.