Cry2 Antibody - C-terminal region (OAAB16491)

• Product Number: OAAB16491
• Name: Cry2 Antibody - C-terminal region (OAAB16491)
• Molecular Weight: 66947 Da
• Isotype: Ig
• NCBI Gene Id: 1408
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 2 mg/ml
• Alias Symbols: HCRY2, PHLL2
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central gears driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Note=Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL
  Tissue Location: Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Cry2.
• Intended Use: For research use only.
• Uniprot ID: Q49AN0
• Protein Name: Cryptochrome-2
• Protein Accession #: NP_001120929.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: CRY2
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC-P
• Image 1: HepG2, Mouse Liver
  
  Western blot analysis of hCry2-R579 (Cat. # OAAB16491) in mouse liver tissue and HepG2 cell line lysates (35ug/lane). CRY2 (arrow) was detected using the purified Pab.

  CRY2 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Image 2: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.