| Product Number |
OAAB16481 |
| Product Page |
www.avivasysbio.com/abcb5-antibody-n-terminal-region-oaab16481.html |
| Name |
ABCB5 Antibody - N-terminal region (OAAB16481) |
| Molecular Weight |
89832 Da |
| Isotype |
Ig |
| NCBI Gene Id |
340273 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Alias Symbols |
ABCB5beta, EST422562, ABCB5alpha |
| Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Description of Target |
Purified Rabbit Polyclonal Antibody (Pab) |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAAB16481 |
| Additional Information |
Precautions: ABCB5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Additional Information |
Function: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas |
| Application Info |
FC: 1:25
WB: 1:2000
WB: 1:1000
FC: 1:10~50 |
| Immunogen |
This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5. |
| Additional Information |
Cellular Location: Cell membrane; Multi-pass membrane protein.
Tissue Location: Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues |
| Uniprot ID |
Q2M3G0 |
| Protein Name |
ATP-binding cassette sub-family B member 5 |
| Protein Accession # |
NP_001157413.1 |
| Gene Symbol |
ABCB5 |
| Predicted Species Reactivity |
Human |
| Application |
WB, FC |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | HL-60, K562, A2058
 | Western blot analysis of ABCB5 Antibody (N-term) (Cat.# OAAB16481) in A375, K562, A2058 and HL-60 cell line lysates (35ug/lane). ABCB5 (arrow) was detected using the purified Pab. |
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| Image 2 | WM852
 | Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# OAAB16481 on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver |
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| Image 3 | SkMel-30
 | Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# OAAB16481 on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver |
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