p27Kip1 Antibody (Phospho-Thr170) (OAAB16425)

Data Sheet
 
Product Number OAAB16425
Product Page www.avivasysbio.com/p27kip1-antibody-phospho-t170-oaab16425.html
Name p27Kip1 Antibody (Phospho-Thr170) (OAAB16425)
Molecular Weight 22073 Da
Isotype Ig
NCBI Gene Id 1027
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols KIP1, MEN4, CDKN4, MEN1B, P27KIP1
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Description of Target Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16425
Additional Information Precautions: p27Kip1 Antibody (Phospho T170) is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry
Application Info DB: 1:500
Immunogen This p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T170 of human p27Kip1.
Additional Information Cellular Location: Nucleus. Cytoplasm. Endosome (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export
Tissue Location: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney
Uniprot ID P46527
Protein Name Cyclin-dependent kinase inhibitor 1B
Protein Accession # NP_004055.1
Gene Symbol CDKN1B
Predicted Species Reactivity Human
Application DB
Predicted Homology Based on Immunogen Sequence Human
Image 1
Phospho-peptide, non-phospho-peptide
Dot blot analysis of p27Kip1 Antibody (Phospho T170) Phospho-specific Pab (Cat. # OAAB16425) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
 

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