IKKB Antibody (Phospho-Ser701) (OAAB16400)

• Product Number: OAAB16400
• Name: IKKB Antibody (Phospho-Ser701) (OAAB16400)
• Molecular Weight: 86690 Da
• Isotype: Ig
• NCBI Gene Id: 16150
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: IKK, IKK-, IKK2, IKK-2, IKK[b, IKKbe, IKK-be, IKK[b], IKKbeta, AI132552, IKK-beta
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: mouse IKKB (Phospho S701) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation (By similarity)
• Application Info: DB: 1:500
• Immunogen: This mouse IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S701 of mouse IKKB.
• Additional Information: Cellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Membrane raft (By similarity). Note=Colocalized with DPP4 in membrane rafts (By similarity)
  Tissue Location: Expressed in liver, kidney and spleen.
• Uniprot ID: O88351
• Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• Protein Accession #: NP_034676.1
• Gene Symbol: Ikbkb
• Predicted Species Reactivity: Mouse
• Application: DB
• Predicted Homology Based on Immunogen Sequence: Mouse
• Image 1: Phospho-peptide, non-phospho-peptide
  
  Dot blot analysis of mouse IKKB Antibody (Phospho S701) Phospho-specific Pab (Cat. # OAAB16400) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.