Product Number |
OAAB16312 |
Product Page |
www.avivasysbio.com/kit-antibody-phospho-y719-oaab16312.html |
Name |
KIT Antibody (Phospho-Tyr719) (OAAB16312) |
Molecular Weight |
109343 Da |
Isotype |
Ig |
NCBI Gene Id |
16590 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
W, Bs, SC, SO, Fdc, Ssm, Gsfs, SCO1, SCO5, SOW3, Tr-k, c-KI, CD117, belly, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3 |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Description of Target |
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB16312 |
Additional Information |
Precautions: Phospho-mouse KIT-Y719 is for research use only and not for use in diagnostic or therapeutic procedures. |
Additional Information |
Function: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1 |
Application Info |
DB: 1:500 |
Immunogen |
This mouse KIT Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y719 of mouse KIT. |
Additional Information |
Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head (By similarity) Tissue Location: Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detecte |
Uniprot ID |
P05532 |
Protein Name |
Mast/stem cell growth factor receptor Kit |
Protein Accession # |
NP_001116205.1 |
Gene Symbol |
Kit |
Predicted Species Reactivity |
Mouse |
Application |
DB |
Predicted Homology Based on Immunogen Sequence |
Mouse |
Image 1 | Phospho-peptide, non-phospho-peptide
| Dot blot analysis of Phospho-mouse KIT-Y719 Antibody Phospho-specific Pab (Cat. # OAAB16312) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml. |
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