LIN28 Antibody (Phospho-Ser134) (OAAB16221)

Data Sheet
 
Product Number OAAB16221
Product Page www.avivasysbio.com/lin28-antibody-phospho-s134-oaab16221.html
Name LIN28 Antibody (Phospho-Ser134) (OAAB16221)
Molecular Weight 22743 Da
Isotype Ig
NCBI Gene Id 79727
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Description of Target Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16221
Additional Information Precautions: Phospho-LIN28- S134 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7 Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation
Application Info IF: 1:100
DB: 1:500
Immunogen This LIN28 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S134 of human LIN28.
Additional Information Cellular Location: Cytoplasm. Nucleus, nucleolus. Note=Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules
Tissue Location: Expressed in embryonic stem cells (ES cells), placenta and testis
Uniprot ID Q9H9Z2
Protein Name Protein lin-28 homolog A
Protein Accession # NP_078950.1
Gene Symbol LIN28A
Predicted Species Reactivity Human
Application IF, DB
Predicted Homology Based on Immunogen Sequence Human
Image 1
Phospho-peptide, non-phospho-peptide
Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab (Cat. # OAAB16221) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Image 2
Human, SY5Y
Human, SY5Y
 

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