LC3B Antibody (Phospho-Thr12) (OAAB16218)

• Product Number: OAAB16218
• Name: LC3B Antibody (Phospho-Thr12) (OAAB16218)
• Molecular Weight: 15 kDa
• Isotype: Ig
• NCBI Gene Id: 81631
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.35 mg/ml
• Alias Symbols: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
  Cellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome Note=LC3-II binds to the autophagic membranes. Localizes also to discrete punctae along the ciliary axoneme (By similarity).
  Tissue Location: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
• Application Info: DB: 1:500
• Immunogen: This LC3B Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T12 of human LC3B.
• Uniprot ID: Q9GZQ8
• Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• Protein Accession #: NP_073729.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: MAP1LC3B
• Predicted Species Reactivity: Human
• Application: DB
• Predicted Homology Based on Immunogen Sequence: Bovine
• Image 1: Phospho-peptide, non-phospho-peptide
  
  Dot blot analysis of Phospho-LC3 (APG8b) - T12 Antibody (Cat.# OAAB16218) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.