Raptor Antibody (Phospho-Ser863) (OAAB16216)

• Product Number: OAAB16216
• Name: Raptor Antibody (Phospho-Ser863) (OAAB16216)
• Molecular Weight: 149038 Da
• Isotype: Ig
• NCBI Gene Id: 57521
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: KOG1, Mip1
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: Raptor participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). It plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. It has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, raptor serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates mTORC1 is activated in response to growth factors or amino acids Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for ciliogenesis.
  Cellular Location: Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.
  Tissue Location: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.
• Application Info: DB: 1:500
• Immunogen: This Raptor Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S863 of human Raptor.
• Intended Use:
  For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q8N122
• Protein Name: Regulatory-associated protein of mTOR
• Protein Accession #: NP_065812.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: RPTOR
• Predicted Species Reactivity: Human
• Application: DB
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Phospho-peptide, non-phospho-peptide
  
  Dot blot analysis of anti-raptor-pS863 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.