EGFR Antibody (Phospho-Tyr1125) (OAAB16202)

• Product Number: OAAB16202
• Name: EGFR Antibody (Phospho-Tyr1125) (OAAB16202)
• Molecular Weight: 134277 Da
• Isotype: Ig
• NCBI Gene Id: 1956
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: Phospho-EGFR-Y1125 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin
• Application Info: DB: 1:500
• Immunogen: This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1125 of human EGFR.
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand
  Tissue Location: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers
• Uniprot ID: P00533
• Protein Name: Epidermal growth factor receptor
• Protein Accession #: NP_005219.2
• Gene Symbol: EGFR
• Predicted Species Reactivity: Human
• Application: DB
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: Phospho-peptide, non-phospho-peptide
  
  Dot blot analysis of Phospho-EGFR-Y1125 Antibody (Cat. # OAAB16202) and EGFR Non Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.