| Product Number |
OAAB16131 |
| Product Page |
www.avivasysbio.com/wee1-antibody-phospho-s123-oaab16131.html |
| Name |
Wee1 Antibody (Phospho-Ser123) (OAAB16131) |
| Molecular Weight |
71597 Da |
| Isotype |
Ig |
| NCBI Gene Id |
7465 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Alias Symbols |
WEE1A, WEE1hu |
| Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Description of Target |
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAAB16131 |
| Additional Information |
Precautions: Phospho-Wee1-S123 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Additional Information |
Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation |
| Application Info |
DB: 1:500
IHC-P: 1:50~100 |
| Immunogen |
This Wee1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S123 of human Wee1. |
| Additional Information |
Cellular Location: Nucleus. |
| Uniprot ID |
P30291 |
| Protein Name |
Wee1-like protein kinase |
| Protein Accession # |
NP_001137448.1 |
| Gene Symbol |
WEE1 |
| Predicted Species Reactivity |
Human |
| Application |
DB |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | Phospho-peptide, non-phospho-peptide
 | Dot blot analysis of anti-Phospho-Wee1-S123 Antibody (Cat. # OAAB16131) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. |
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