RB1 Antibody (Phospho-Ser811) (OAAB16112)

Data Sheet
 
Product Number OAAB16112
Product Page www.avivasysbio.com/rb-antibody-phospho-s811-oaab16112.html
Name RB1 Antibody (Phospho-Ser811) (OAAB16112)
Molecular Weight 106159 Da
Isotype Ig
NCBI Gene Id 5925
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols RB, pRb, OSRC, pp110, p105-Rb, PPP1R130, p110-RB1
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Description of Target Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16112
Additional Information Precautions: Phospho-Rb-S811 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Additional Information Function: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1 Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity
Application Info DB: 1:500
IHC-P: 1:50~100
IF: 1:10~50
Immunogen This Rb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S811 of human Rb.
Additional Information Cellular Location: Nucleus.
Tissue Location: Expressed in the retina.
Uniprot ID P06400
Protein Name Retinoblastoma-associated protein
Protein Accession # NP_000312.2
Gene Symbol RB1
Predicted Species Reactivity Human
Application DB, IHC, IF
Predicted Homology Based on Immunogen Sequence Human
Image 1
hepatocarcinoma
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Image 2
Phospho-peptide, non-phospho-peptide
Dot blot analysis of anti-hRb-S811 Phospho-specific Pab (Cat. # OAAB16112) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
Image 3
HepG2, Nuclear
Confocal immunofluorescent analysis of Phospho-Rb-S811 Antibody(Cat# OAAB16112) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
 

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