RAD9 Antibody (Phospho-Ser328) (OAAB16099)

• Product Number: OAAB16099
• Name: RAD9 Antibody (Phospho-Ser328) (OAAB16099)
• Molecular Weight: 42547 Da
• Isotype: Ig
• NCBI Gene Id: 5883
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: RAD9
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: Phospho-RAD9-S328 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex
• Application Info: DB: 1:500
  WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This RAD9 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S328 of human RAD9.
• Additional Information: Cellular Location: Nucleus.
• Uniprot ID: Q99638
• Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Accession #: NP_001230153.1
• Gene Symbol: RAD9A
• Predicted Species Reactivity: Human
• Application: DB, WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: breast carcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: HL60, Rad9, Y79
  
  The anti-Phospho-Rad9-S328 Pab (Cat. # OAAB16099) is used in Western blot to detect Phospho-Rad9-S328 in HL60 (left) and Y79 (right) tissue lysates.
• Image 3: Phospho-peptide, non-phospho-peptide
  
  Dot blot analysis of anti-Phospho-Rad9-S328 Antibody (Cat.# OAAB16099) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.