Product Number |
OAAB16097 |
Product Page |
www.avivasysbio.com/rad9-antibody-phospho-s272-oaab16097.html |
Name |
RAD9 Antibody (Phospho-Ser272) (OAAB16097) |
Molecular Weight |
42547 Da |
Isotype |
Ig |
NCBI Gene Id |
5883 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
RAD9 |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Description of Target |
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB16097 |
Additional Information |
Precautions: Phospho-RAD9-S272 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Additional Information |
Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex |
Application Info |
WB: 1:1000 IHC-P: 1:10~100 |
Immunogen |
This RAD9 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S272 of human RAD9. |
Additional Information |
Cellular Location: Nucleus. |
Uniprot ID |
Q99638 |
Protein Name |
Cell cycle checkpoint control protein RAD9A |
Protein Accession # |
NP_001230153.1 |
Gene Symbol |
RAD9A |
Predicted Species Reactivity |
Human |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Human |
Image 1 | hepatocarcinoma
| Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
| Image 2 | Hela, HL60, Rad9
| The anti-Phospho-Rad9-S272 Pab (Cat. # OAAB16097) is used in Western blot to detect Phospho-Rad9-S272 in HL60 (left), Hela(middle), and Y79(right) tissue lysates. |
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