p53 Antibody (Phospho-Thr18) (OAAB16093)

Data Sheet
 
Product Number OAAB16093
Product Page www.avivasysbio.com/p53-antibody-phospho-t18-oaab16093.html
Name p53 Antibody (Phospho-Thr18) (OAAB16093)
Molecular Weight 44 kDa
Isotype Ig
NCBI Gene Id 7157
Host Rabbit
Clonality Polyclonal
Concentration 0.35 mg/ml
Alias Symbols P53, BCC7, LFS1, BMFS5, TRP53
Product Format Liquid PBS with 0.09% (W/V) sodium azide.
Description of Target Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.
Reconstitution and Storage Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB16093
Additional Information Cellular Location: Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Note=Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Translocates to mitochondria upon oxidative stress Isoform 2: Nucleus. Cytoplasm. Note=Localized mainly in the nucleus with minor staining in the cytoplasm Isoform 4: Nucleus. Cytoplasm. Note=Predominantly nucle
Tissue Location: Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed
Application Info DB: 1:500
WB: 1:1000
IHC-P: 1:50~100
Immunogen This p53 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T18 of human p53.
Uniprot ID P04637
Protein Name Cellular tumor antigen p53
Protein Accession # NP_000537.3
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Tested Species Reactivity Human, Mouse
Gene Symbol TP53
Application DB, WB, IHC-P
Predicted Homology Based on Immunogen Sequence Monkey, Pig, Rabbit
Image 1
nitrocellulose membrane
Dot blot analysis of anti-hp53-T18 Phospho-specific Pab (Cat. #OAAB16093) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
Image 2
human cancer tissue
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Image 3
A2058, Ramos, mouse lung, mouse testis, , HL60 cell
Western blot analysis of anti-Phospho-p53-T18 Pab (cat# OAAB16093) in, from left to right, A2058, Ramos, mouse lung, mouse testis, and HL60 cell line lysates. Phospho-p53-T18(arrow) was detected using the purified Pab.
 

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