MAPKAPK5 Antibody (Phospho-Ser93) (OAAB16064)

• Product Number: OAAB16064
• Name: MAPKAPK5 Antibody (Phospho-Ser93) (OAAB16064)
• Molecular Weight: 54220 Da
• Isotype: Ig
• NCBI Gene Id: 8550
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: MK5, MK-5, PRAK, MAPKAP-K5
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: Phospho-MAPKAPK5-S93 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras- induced senescence and phosphorylating p53/TP53. Involved in post- transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Immunogen: This MAPKAPK5 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S93 of human MAPKAPK5.
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export
  Tissue Location: Expressed ubiquitously.
• Uniprot ID: Q8IW41
• Protein Name: MAP kinase-activated protein kinase 5
• Protein Accession #: NP_003659.2
• Gene Symbol: MAPKAPK5
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 2: MAPKAPK5, Mouse Heart
  
  The anti-Phospho-MAPKAPK5-S93 Pab (Cat. # OAAB16064) is used in Western blot to detect Phospho-MAPKAPK5-S93 in mouse heart tissue lysate. PAD4(arrow) was detected using the purified Pab.