PI3KC3 Antibody (Phospho-S34) (OAAB14061)

• Product Number: OAAB14061
• Name: PI3KC3 Antibody (Phospho-S34) (OAAB14061)
• Molecular Weight: 101549 Da
• Isotype: Ig
• NCBI Gene Id: 5289
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: VPS34, Vps34, hVps34
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: PI3KC3 Antibody (S34) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes Required for the abcission step in cytokinesis. Required for transport from early to late endosomes
• Application Info: IF: 1:200
  WB: 1:1000
  IHC-P: 1:10~50
  IHC-P: 1:100
• Immunogen: This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-39 amino acids from human PI3KC3.
• Additional Information: Cellular Location: Midbody. Late endosome.
  Tissue Location: Ubiquitously expressed, with a highest expression in skeletal muscle
• Uniprot ID: Q8NEB9
• Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• Protein Accession #: NP_002638.2
• Gene Symbol: PIK3C3
• Predicted Species Reactivity: Human, Mouse, Rat, Pig
• Application: IF, WB, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Pig; Rat
• Image 1: Hela cell
  
  Western blot analysis of PI3KC3 (S34) (Cat. #OAAB14061) in Hela cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.
• Image 2: human skeletal muscle tissue
  
  Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody (S34), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: U251 cell
  
  Fluorescent image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine (50 ?M, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with OAAB14061 PI3KC3 (S34) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.