| Product Number |
OAAB13518 |
| Product Page |
www.avivasysbio.com/cleaved-lc3b-antibody-oaab13518.html |
| Name |
Cleaved LC3B Antibody (OAAB13518) |
| Molecular Weight |
14688 |
| Isotype |
Ig |
| NCBI Gene Id |
81631 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.39 mg/ml |
| Gene Full Name |
microtubule associated protein 1 light chain 3 beta |
| Alias Symbols |
ATG8F;Autophagy-related protein LC3 B;autophagy-related ubiquitin-like modifier LC3 B;LC3B;MAP1 light chain 3-like protein 2;MAP1A/1BLC3;MAP1A/MAP1B LC3 B;MAP1A/MAP1B light chain 3 B;MAP1LC3B-a;Microtubule-associated protein 1 light chain 3 beta;microtubule-associated proteins 1A/1B light chain 3B. |
| Product Format |
Liquid. PBS with 0.09% (W/V) sodium azide. |
| Description of Target |
Microtubule-associated proteins 1A/1B light chain 3B, microtubule-associated proteins 1A/1B light chain 3B [Homo sapiens] |
| Reconstitution and Storage |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for Cleaved LC3B Antibody (OAAB13518) |
| Additional Information |
Cellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome Note=LC3-II binds to the autophagic membranes. Localizes also to discrete punctae along the ciliary axoneme.
Tissue Location: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. |
| Application Info |
IF: 1:10~50
ICC: 1:10~50 |
| Immunogen |
This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B. |
| Uniprot ID |
https://www.uniprot.org/uniprot/?query=O41515+OR+Q9GZQ8 |
| Protein Name |
NP_073729.1, O41515, Q9GZQ8 |
| Protein Accession # |
https://www.ncbi.nlm.nih.gov/protein/NP_073729.1 |
| Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
MAP1LC3B |
| Predicted Species Reactivity |
Bovine, Human, Mouse |
| Application |
Immunocytochemistry, Immunofluorescence |
| Image 1 | Mouse
 | Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody (Cat OAAB13518) detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University. |
| | Image 2 | U251
 | U251 |
|
|
