MAP1LC3B Antibody (OAAB13483)

• Product Number: OAAB13483
• Name: MAP1LC3B Antibody (OAAB13483)
• Molecular Weight: 14688 Da
• Isotype: Ig
• NCBI Gene Id: 81631
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Precautions: LC3 Antibody (APG8A/B) is for research use only and not for use in diagnostic or therapeutic procedures.
• Additional Information: Function: Involved in formation of autophagosomal vacuoles (autophagosomes)
• Application Info: WB: 1:500
  IHC-P: 1:50~100
• Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from human LC3.
• Additional Information: Cellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes
  Tissue Location: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
• Uniprot ID: Q9GZQ8
• Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• Publications:

  NKCT1 (purified Naja kaouthia protein toxin) conjugated gold nanoparticles induced Akt/mTOR inactivation mediated autophagic and caspase 3 activated apoptotic cell death in leukemic cell. Toxicon. 121, 86-97 (2016). 27527270

• Protein Accession #: NP_073729.1
• Gene Symbol: MAP1LC3B
• Predicted Species Reactivity: Human, Mouse, Rat, Cow
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat
• Image 1: Y79
  
  Western blot analysis of anti-Autophagy LC3 Antibody (Cat.# OAAB13483) in Y79 cell line lysates (35ug/lane). APG8 (arrow) was detected using the purified Pab.
• Image 2: hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
• Image 3: Mouse Lung
  
  Western blot analysis of anti-Autophagy LC3 Antibody (Cat.# OAAB13483) in mouse lung tissue lysates (35ug/lane). APG8 (arrow) was detected using the purified Pab.