KRAS Antibody - C - terminal region (OAAB11771)

Data Sheet
 
Product Number OAAB11771
Product Page www.avivasysbio.com/kras-antibody-c-terminal-region-oaab11771.html
Name KRAS Antibody - C - terminal region (OAAB11771)
Molecular Weight 22 kDa
Isotype Ig
NCBI Gene Id 3845
Host Rabbit
Clonality Polyclonal
Concentration Varies by lot. See vial for concentration.
Alias Symbols NS, NS3, OES, CFC2, RALD, K-Ras, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras 2, C-K-RAS, c-Ki-ras, c-Ki-ras2
Product Format Liquid. PBS with 0.09% (W/V) sodium azide.
Description of Target This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
Reconstitution and Storage Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB11771
Additional Information Function: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner
Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol.
Application Info WB: 1:2000
IF: 1:10~50
FC: 1:10~50
Immunogen This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the C-terminal region of human KRAS.
Intended Use For research use only and not for use in diagnostic or therapeutic procedures.
Uniprot ID P01116
Protein Name GTPase KRas
Protein Accession # NP_004976.2
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Gene Symbol KRAS
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IF, FC,
Predicted Homology Based on Immunogen Sequence Rat
Image 1
WiDr
Confocal immunofluorescent analysis of KRAS Antibody (C-term)(Cat#OAAB11771) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Image 2
Hela
KRAS Antibody (C-term) (Cat. #OAAB11771) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

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