Product Number |
OAAB11771 |
Product Page |
www.avivasysbio.com/kras-antibody-c-terminal-region-oaab11771.html |
Name |
KRAS Antibody - C - terminal region (OAAB11771) |
Molecular Weight |
22 kDa |
Isotype |
Ig |
NCBI Gene Id |
3845 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Varies by lot. See vial for concentration. |
Alias Symbols |
NS, NS3, OES, CFC2, RALD, K-Ras, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras 2, C-K-RAS, c-Ki-ras, c-Ki-ras2 |
Product Format |
Liquid. PBS with 0.09% (W/V) sodium azide. |
Description of Target |
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
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Reconstitution and Storage |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB11771 |
Additional Information |
Function: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. |
Application Info |
WB: 1:2000 IF: 1:10~50 FC: 1:10~50 |
Immunogen |
This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the C-terminal region of human KRAS. |
Intended Use |
For research use only and not for use in diagnostic or therapeutic procedures. |
Uniprot ID |
P01116 |
Protein Name |
GTPase KRas |
Protein Accession # |
NP_004976.2 |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Gene Symbol |
KRAS |
Predicted Species Reactivity |
Human, Mouse, Rat |
Application |
WB, IF, FC, |
Predicted Homology Based on Immunogen Sequence |
Rat |
Image 1 | WiDr
| Confocal immunofluorescent analysis of KRAS Antibody (C-term)(Cat#OAAB11771) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
| Image 2 | Hela
| KRAS Antibody (C-term) (Cat. #OAAB11771) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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