Mouse Pkmyt1 Antibody - N-terminal region (OAAB10376)

• Product Number: OAAB10376
• Name: Mouse Pkmyt1 Antibody - N-terminal region (OAAB10376)
• Molecular Weight: 54 kDa
• Isotype: Ig
• NCBI Gene Id: 268930
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: Myt1, AW209059, 6230424P17
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Description of Target: Pkmyt1 acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity)
  Cellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). Golgi apparatus membrane; Peripheral membrane protein (By similarity)
• Application Info: WB: 1:1000
• Immunogen: This Mouse Pkmyt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-143 amino acids from the N-terminal region of mouse Pkmyt1.
• Uniprot ID: Q9ESG9
• Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Protein Accession #: NP_075545.2
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: Pkmyt1
• Predicted Species Reactivity: Human, Mouse
• Application: WB
• Image 1: Mouse Heart
  
  Mouse Pkmyt1 Antibody (N-term) (Cat. #OAAB10376) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).
• Image 2: HL-60
  
  Mouse Pkmyt1 Antibody (N-term) (Cat. #OAAB10376) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow)
• Image 3: 293
  
  Western blot analysis of Pkmyt1 (arrow) using rabbit polyclonal Mouse Pkmyt1 Antibody (N-term) (Cat. #OAAB10376). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.