Product Number |
OAAB09358 |
Product Page |
www.avivasysbio.com/gnas-antibody-c-terminal-region-oaab09358.html |
Name |
GNAS Antibody - C-terminal region (OAAB09358) |
Molecular Weight |
44 kDa |
Isotype |
Rabbit IgG |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
GNAS |
Product Format |
Liquid PBS with 0.09% (W/V) sodium azide. |
Description of Target |
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for OAAB09358 |
Application Info |
WB: 1:1000 IHC-P: 1:10~50 IF: 1:10~50 FC: 1:10~50 |
Immunogen |
This GNAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-315 amino acids from the C-terminal region of human GNAS. |
Uniprot ID |
Q5FWY2 |
Protein Name |
GNAS complex locus EMBL AAH89157.2 |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Predicted Species Reactivity |
Human |
Application |
WB, IHC-P, IF, FC |
Predicted Homology Based on Immunogen Sequence |
Mouse, Rat, Bovine, Hamster, Pig |
Image 1 | Human Pancreas
| GNAS Antibody (C-term) (Cat. #OAAB09358)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GNAS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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Image 2 | 293, NCI-H292
| GNAS Antibody (C-term) (Cat. #OAAB09358) western blot analysis in 293,NCI-H292 cell line lysates (35ug/lane).This demonstrates the GNAS antibody detected the GNAS protein (arrow). |
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Image 3 | 293
| Confocal immunofluorescent analysis of GNAS Antibody (C-term)(Cat#OAAB09358) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
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Image 4 | 293
| GNAS Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Image 5 | 293
| Western blot analysis of GNAS (arrow) using rabbit polyclonal GNAS Antibody (C-term) (Cat. #OAAB09358). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene. |
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