GNAS Antibody - C-terminal region (OAAB09358)

Data Sheet
 
Product Number OAAB09358
Product Page www.avivasysbio.com/gnas-antibody-c-terminal-region-oaab09358.html
Name GNAS Antibody - C-terminal region (OAAB09358)
Molecular Weight 44 kDa
Isotype Rabbit IgG
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols GNAS
Product Format Liquid PBS with 0.09% (W/V) sodium azide.
Description of Target Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for OAAB09358
Application Info WB: 1:1000
IHC-P: 1:10~50
IF: 1:10~50
FC: 1:10~50
Immunogen This GNAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-315 amino acids from the C-terminal region of human GNAS.
Uniprot ID Q5FWY2
Protein Name GNAS complex locus EMBL AAH89157.2
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Predicted Species Reactivity Human
Application WB, IHC-P, IF, FC
Predicted Homology Based on Immunogen Sequence Mouse, Rat, Bovine, Hamster, Pig
Image 1
Human Pancreas
GNAS Antibody (C-term) (Cat. #OAAB09358)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GNAS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Image 2
293, NCI-H292
GNAS Antibody (C-term) (Cat. #OAAB09358) western blot analysis in 293,NCI-H292 cell line lysates (35ug/lane).This demonstrates the GNAS antibody detected the GNAS protein (arrow).
Image 3
293
Confocal immunofluorescent analysis of GNAS Antibody (C-term)(Cat#OAAB09358) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Image 4
293
GNAS Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Image 5
293
Western blot analysis of GNAS (arrow) using rabbit polyclonal GNAS Antibody (C-term) (Cat. #OAAB09358). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene.
 

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