ANKH Antibody - C-terminal region (OAAB06341)

• Product Number: OAAB06341
• Name: ANKH Antibody - C-terminal region (OAAB06341)
• Molecular Weight: 54 kDa
• Isotype: Rabbit Ig
• NCBI Gene Id: 56172
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Varies by lot. See vial for concentration.
• Gene Full Name: ANKH inorganic pyrophosphate transport regulator
• Description:
  View product citations for ANKH Antibody - C-terminal region (OAAB06341) on CiteAb
• Alias Symbols: ANK;ankylosis, progressive homolog;CCAL2;CMDJ;CPPDD;Fn54 protein;HANK;MANK;progressive ankylosis protein homolog;SLC62A1.
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Membrane; Multi-pass membrane protein
  Tissue Location: Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
  Optimal dilutions should be determined by the end user.
• Other Applications Image 1 Data: ANKH Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: https://www.uniprot.org/uniprot/?query=P58366+OR+Q9HCJ1+OR+Q9JHZ2
• Protein Name: NP_473368.1, P58366, Q9HCJ1, Q9JHZ2
• Publications:

  Szeri, et al. The membrane protein ANKH is crucial for bone mechanical performance by mediating cellular export of citrate and ATP. PLoS Genetics 2020 Jul 8 16(7):e1008884. 32639996

• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_473368.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: ANKH
• Predicted Species Reactivity: Human, Mouse, Rat
• Application: Flow cytometry, Immunohistochemistry-Paraffin, Western blot
• Image 1: mouse cerebellum tissue
  
  Western blot analysis of ANKH Antibody (C-term) (Cat. #OAAB06341) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab.
• Image 2: brain tissue
  
  ANKH Antibody (C-term) (Cat. #OAAB06341) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 3: K562 cell
  
  ANKH Antibody (C-term) (Cat. #OAAB06341) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.