Product Number |
OAAB05777 |
Product Page |
www.avivasysbio.com/dek-antibody-c-terminal-region-oaab05777.html |
Name |
DEK Antibody - C-terminal region (OAAB05777) |
Molecular Weight |
42674 Da |
Isotype |
Rabbit Ig |
NCBI Gene Id |
7913 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
D6S231E |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-DEK antibody - OAAB05777 |
Additional Information |
Function: Involved in chromatin organization.
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Additional Information |
Cellular Location: Nucleus. Note=Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei Tissue Location: Ubiquitous. Expressed at relatively high levels |
Application Info |
WB: 1:1000 IHC-P: 1:50~100 FC: 1:10~50 IF: 1:10~50 |
Immunogen |
This DEK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human DEK. |
Uniprot ID |
P35659 |
Protein Name |
Protein DEK |
Publications |
Clawson, G. A., Bui, V., Xin, P., Wang, N. & Pan, W. Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins. J. Cell. Biochem. 105, 81-8 (2008). 18452160
Privette Vinnedge, L. M. et al. The human DEK oncogene stimulates beta-catenin signaling, invasion and mammosphere formation in breast cancer. Oncogene 30, 2741-52 (2011). 21317931 |
Protein Accession # |
NP_001128181.1 |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Gene Symbol |
DEK |
Predicted Species Reactivity |
Human |
Application |
WB, IHC, IF, FC |
Predicted Homology Based on Immunogen Sequence |
Human |
Image 1 | Human Testis
| DEK Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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Image 2 | WiDr
| DEK Antibody (C-term) (Cat. #OAAB05777) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Image 3 | HeLa
| Confocal immunofluorescent analysis of DEK Antibody (C-term) (Cat#OAAB05777) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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Image 4 | U-251 MG, PC-3
| Western blot analysis of lysates from U-251 MG, PC-3 cell line (from left to right), using DEK Antibody (C-term)(Cat. #OAAB05777). OAAB05777 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
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