DEK Antibody - C-terminal region (OAAB05777)

Data Sheet
 
Product Number OAAB05777
Product Page www.avivasysbio.com/dek-antibody-c-terminal-region-oaab05777.html
Name DEK Antibody - C-terminal region (OAAB05777)
Molecular Weight 42674 Da
Isotype Rabbit Ig
NCBI Gene Id 7913
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols D6S231E
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-DEK antibody - OAAB05777
Additional Information Function:
Involved in chromatin organization.
Additional Information Cellular Location: Nucleus. Note=Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei
Tissue Location: Ubiquitous. Expressed at relatively high levels
Application Info WB: 1:1000
IHC-P: 1:50~100
FC: 1:10~50
IF: 1:10~50
Immunogen This DEK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human DEK.
Uniprot ID P35659
Protein Name Protein DEK
Publications

Clawson, G. A., Bui, V., Xin, P., Wang, N. & Pan, W. Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins. J. Cell. Biochem. 105, 81-8 (2008). 18452160

Privette Vinnedge, L. M. et al. The human DEK oncogene stimulates beta-catenin signaling, invasion and mammosphere formation in breast cancer. Oncogene 30, 2741-52 (2011). 21317931

Protein Accession # NP_001128181.1
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Gene Symbol DEK
Predicted Species Reactivity Human
Application WB, IHC, IF, FC
Predicted Homology Based on Immunogen Sequence Human
Image 1
Human Testis
DEK Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Image 2
WiDr
DEK Antibody (C-term) (Cat. #OAAB05777) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Image 3
HeLa
Confocal immunofluorescent analysis of DEK Antibody (C-term) (Cat#OAAB05777) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Image 4
U-251 MG, PC-3
Western blot analysis of lysates from U-251 MG, PC-3 cell line (from left to right), using DEK Antibody (C-term)(Cat. #OAAB05777). OAAB05777 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
 

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