HMGA1 Antibody - C-terminal region (OAAB05695)

• Name: HMGA1 Antibody - C-terminal region (OAAB05695)
• Molecular Weight: 11676
• Isotype: Rabbit Ig
• NCBI Gene Id: 100689040|111241|117062|15361|3159
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: AL023995, high mobility group AT-hook I, related sequence 1, high mobility group AT-hook protein 1, high mobility group protein A1, high mobility group protein HMG-I/HMG-Y, high mobility group protein I, high mobility group protein R, high mobility group-I protein, Hmg, Hmg-i, HMG-I(Y, HMG-I(Y), HMG-R, HMG-Y, Hmga, Hmga1-, Hmga1-rs1, HMGA1A, Hmga1b, Hmgi, Hmgi-, Hmgi-rs1, HMGI(Y, HMGIY, Hmgy, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y.
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions (By similarity).|HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
  Cellular Location: Nucleus. Chromosome.
  
• Application Info: WB: 1:1000
  FC: 1:10~50
  IF: 1:10~50
• Other Applications Image 1 Data: HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.
• Intended Use: HMGA1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: P17095|P17096|Q8K585|Q9QXP3
• Protein Name: High mobility group protein HMG-I/HMG-Y|high mobility group protein HMG-I/HMG-Y isoform a [Homo sapiens]|high mobility group protein HMG-I/HMG-Y isoform b [Homo sapiens]
• Sample Type Confirmation:

  HMGA1 is supported by BioGPS gene expression data to be expressed in MDA-MB231

• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_002122.1,NP_665906.1,NP_665908.1,NP_665909.1,NP_665910.1,NP_665912.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: HMGA1
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Application: FC,IF,WB
• Predicted Homology Based on Immunogen Sequence: Hamster; Human; Mouse; Rat
• Image 1: MCF-7 cell
  
  HMGA1 Antibody (C-term) (Cat. #OAAB05695) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 2: A549 cell
  
  Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term)(Cat#OAAB05695).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).HMGA1 immunoreactivity is localized to Nucleus significantly.
• Image 3: K562 cell
  
  HMGA1 Antibody (C-term) (Cat. #OAAB05695) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).