EXTL3 Antibody - N-terminal region (OAAB05635)

• Name: EXTL3 Antibody - N-terminal region (OAAB05635)
• Molecular Weight: 104749
• Isotype: Rabbit Ig
• NCBI Gene Id: 2137
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: BOTV, epididymis secretory sperm binding protein, exostoses (multiple)-like 3, exostosin tumor-like 3, exostosin-like 3, EXT-related 1, EXT-related protein 1, EXTL1L, EXTR1, glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, hereditary multiple exostoses gene isolog, ISDNA, Multiple exostosis-like protein 3, putative tumor suppressor protein EXTL3, reg receptor, REGR, RPR.
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: Glycosyltransferase which regulates the biosynthesis of heparan sulfate (HS). Important for both skeletal development and hematopoiesis, through the formation of HS proteoglycans (HSPGs) (PubMed:28132690, PubMed:28148688). Required for the function of REG3A in regulating keratinocyte proliferation and differentiation (PubMed:22727489).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Probable glycosyltransferase (By similarity). Required for the function of REG3A in regulating keratinocyte proliferation and differentiation.
  Cellular Location: Endoplasmic reticulum membrane; Single-pass type II membrane protein
  Tissue Location Ubiquitous. Expressed in keratinocytes.
  
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: EXTL3 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This EXTL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-72 amino acids from the N-terminal region of human EXTL3.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
  
• Uniprot ID: O43909
• Protein Name: Exostosin-like 3|exostosin-like 3 [Homo sapiens]
• Sample Type Confirmation:

  EXTL3 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB435

• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001431.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: EXTL3
• Predicted Species Reactivity: Human
• Application: FC,IHC-P,WB
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: MDA-MB435
  
  Western blot analysis of EXTL3 Antibody (N-term) in MDA-MB435, MDA-MB468 cell lysates (35ug/lane). EXTL3 (arrow) was detected using the purified Pab.

  EXTL3 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB435

• Image 2: mouse testis
  
  EXTL3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EXTL3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 3: MDA-MB435
  
  EXTL3 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.