SPG20 Antibody - N-terminal region (OAAB05628)

• Product Number: OAAB05628
• Name: SPG20 Antibody - N-terminal region (OAAB05628)
• Molecular Weight: 72833 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 23111
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: SPG20, TAHCCP1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: SPG20 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This SPG20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human SPG20.
• Additional Information: Description: This SPG20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24~54 amino acids from the N-terminal region of human SPG20.
• Uniprot ID: Q8N0X7
• Protein Name: Spartin
• Sample Type Confirmation:

  SPG20 is supported by BioGPS gene expression data to be expressed in NCI-H460

• Protein Accession #: NP_001135766.1
• Gene Symbol: SPG20
• Predicted Species Reactivity: Human
• Application: WB, FC, IHC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: NCI-H460
  
  Western blot analysis of SPG20 Antibody (N-term) in NCI-H460 cell lysates (35ug/lane). SPG20 (arrow) was detected using the purified Pab.

  SPG20 is supported by BioGPS gene expression data to be expressed in NCI-H460

• Image 2: human brain
  
  Formalin-fixed and paraffin-embedded human brain tissue reacted with SPG20 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: NCI-H460
  
  SPG20 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.