DTX1 Antibody - middle region (OAAB05518)

• Name: DTX1 Antibody - middle region (OAAB05518)
• Molecular Weight: 67368
• Isotype: Rabbit Ig
• NCBI Gene Id: 14357|1840
• Host: Rabbit
• Clonality: Polyclonal
• Alias Symbols: deltex 1 homolog, deltex 1, E3 ubiquitin ligase, deltex homolog 1, deltex1, E3 ubiquitin-protein ligase DTX1, fractionated x-irradiation induced transcript 1, FXI-T1, Fxit, Fxit1, hDx-1, mDTX1, mKIAA4160, protein deltex-1, RING-type E3 ubiquitin transferase DTX1, RNF140.
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1.|Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T- cell development, suggesting that it can antagonize NOTCH1.
  Cellular Location: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear
  Tissue Location Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells.
  
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Immunogen: This DTX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the Central region of human DTX1.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q61010|Q86Y01
• Protein Name: E3 ubiquitin-protein ligase DTX1|E3 ubiquitin-protein ligase DTX1 [Homo sapiens]
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004407.2
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: DTX1
• Predicted Species Reactivity: Human|Mouse
• Application: FC,IHC-P,WB
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Hela
  
  Western blot analysis of DTX1 Antibody (Center) in Hela cell lysates (35ug/lane). DTX1 (arrow) was detected using the purified Pab.
• Image 2: Human lymph
  
  Formalin-fixed and paraffin-embedded human lymph tissue reacted with DTX1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: Jurkat cells
  
  DTX1 Antibody (Center) (OAAB05518) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.