GLS Antibody - C-terminal region (OAAB05406)

• Product Number: OAAB05406
• Name: GLS Antibody - C-terminal region (OAAB05406)
• Molecular Weight: 65,460 Da Isoform 3 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 2744
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: GAC, GAM, KGA, GLS1, AAD20, DEE71, GDPAG, CASGID, EIEE71
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: IF: 1:25
  IHC-P: 1:25
  FC: 1:25
  WB: 1:2000
• Other Applications Image 1 Data: GLS Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
• Uniprot ID: O94925
• Protein Name: Glutaminase kidney isoform, mitochondrial
• Protein Accession #: NP_001243239.1
• Gene Symbol: GLS
• Predicted Species Reactivity: Human, Mouse
• Application: WB, FC, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Hela cell
  
  Confocal immunofluorescent analysis of GLS Antibody (C-term) (Cat. #OAAB05406) with Hela cell followed by Alexa Fluor®489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
• Image 2: HepG2 cell
  
  GLS Antibody (C-term) (Cat. #OAAB05406) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 3: mouse liver tissue
  
  Western blot analysis of GLS Antibody (C-term) (Cat. #OAAB05406) in mouse liver tissue lysates (35ug/lane). GLS (arrow) was detected using the purified Pab.
• Image 4: mouse brain tissue
  
  GLS Antibody (C-term) (Cat. #OAAB05406) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.