| Product Number |
OAAB05353 |
| Product Page |
www.avivasysbio.com/bmi1-antibody-oaab05353.html |
| Name |
BMI1 Antibody (OAAB05353) |
| Molecular Weight |
36949 Da |
| Isotype |
Rabbit Ig |
| NCBI Gene Id |
648 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Alias Symbols |
PCGF4, RNF51, FLVI2/BMI1, flvi-2/bmi-1 |
| Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-BMI1 antibody - OAAB05353 |
| Application Info |
IF: 1:25
FC: 1:25
WB: 1:8000
WB: 1:2000
IHC-P: 1:10~50 |
| Other Applications Image 1 Data |
BMI1 Antibody flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
| Immunogen |
This BMI1 antibody is generated from rabbits immunized with BMI1 recombinant protein. |
| Uniprot ID |
P35226 |
| Protein Name |
Polycomb complex protein BMI-1 |
| Protein Accession # |
NP_001190991.1 |
| Gene Symbol |
BMI1 |
| Predicted Species Reactivity |
Human |
| Application |
WB, IHC, FC |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | human breast carcinoma
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with BMI1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
|
| Image 2 | MCF7 cell
 | Western blot analysis of BMI1 Antibody (Cat. #OAAB05353) in MCF7 cell line lysates (35ug/lane). BMI1 (arrow) was detected using the purified Pab. |
|
| Image 3 | MCF-7 cell
 | Confocal immunofluorescent analysis of BMI1 Antibody(Cat#OAAB05353) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). |
|
| Image 4 | MCF-7 cell
 | BMI1 Antibody (Cat. #OAAB05353) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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