Product Number |
OAAB05155 |
Product Page |
www.avivasysbio.com/atp5o-antibody-n-terminal-region-oaab05155.html |
Name |
ATP5O Antibody - N-terminal region (OAAB05155) |
Molecular Weight |
23277 Da |
Isotype |
Rabbit Ig |
NCBI Gene Id |
539 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Alias Symbols |
ATPO, OSCP, ATP5O, HMC08D05 |
Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-ATP5O antibody - OAAB05155 |
Application Info |
WB: 1:1000 IHC-P: 1:50~100 IF: 1:10~50 FC: 1:10~50 |
Other Applications Image 1 Data |
Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Other Applications Image 2 Data |
Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI |
Immunogen |
This ATP5O antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-64 amino acids from the N-terminal region of human ATP5O. |
Uniprot ID |
P48047 |
Protein Name |
ATP synthase subunit O, mitochondrial |
Sample Type Confirmation |
ATP5O is strongly supported by BioGPS gene expression data to be expressed in HEK293 |
Protein Accession # |
NP_001688.1 |
Gene Symbol |
ATP5O |
Predicted Species Reactivity |
Human |
Application |
WB, IHC, IF, FC |
Predicted Homology Based on Immunogen Sequence |
Human |
Image 1 | 293
| Western blot analysis of ATP5O Antibody (N-term) in 293 cell lysates (35ug/lane). ATP5O (arrow) was detected using the purified Pab. ATP5O is strongly supported by BioGPS gene expression data to be expressed in HEK293 |
|
Image 2 | Human Skeletal Muscle
| Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
|
Image 3 | 293
| Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
|
Image 4 | Human Skeletal Muscle
| Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI |
|