ATP5O Antibody - N-terminal region (OAAB05155)

Data Sheet
 
Product Number OAAB05155
Product Page www.avivasysbio.com/atp5o-antibody-n-terminal-region-oaab05155.html
Name ATP5O Antibody - N-terminal region (OAAB05155)
Molecular Weight 23277 Da
Isotype Rabbit Ig
NCBI Gene Id 539
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols ATPO, OSCP, ATP5O, HMC08D05
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ATP5O antibody - OAAB05155
Application Info WB: 1:1000
IHC-P: 1:50~100
IF: 1:10~50
FC: 1:10~50
Other Applications Image 1 Data Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Other Applications Image 2 Data Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI
Immunogen This ATP5O antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-64 amino acids from the N-terminal region of human ATP5O.
Uniprot ID P48047
Protein Name ATP synthase subunit O, mitochondrial
Sample Type Confirmation

ATP5O is strongly supported by BioGPS gene expression data to be expressed in HEK293

Protein Accession # NP_001688.1
Gene Symbol ATP5O
Predicted Species Reactivity Human
Application WB, IHC, IF, FC
Predicted Homology Based on Immunogen Sequence Human
Image 1
293
Western blot analysis of ATP5O Antibody (N-term) in 293 cell lysates (35ug/lane). ATP5O (arrow) was detected using the purified Pab.

ATP5O is strongly supported by BioGPS gene expression data to be expressed in HEK293

Image 2
Human Skeletal Muscle
Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Image 3
293
Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Image 4
Human Skeletal Muscle
Immunofluorescence analysis of ATP5O Antibody (N-term) with paraffin-embedded human skeletal muscle. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI
 

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