IFNB1 Antibody - N-terminal region (OAAB05131)

• Product Number: OAAB05131
• Name: IFNB1 Antibody - N-terminal region (OAAB05131)
• Molecular Weight: 22294 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 3456
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: IFB, IFF, IFNB, IFN-beta
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: FC: 1:25
  WB: 1:2000
  WB: 1:1000
  IHC-P: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of HepG2 cells using IFNB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This IFNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of human IFNB1.
• Uniprot ID: P01574
• Protein Name: Interferon beta
• Publications:

  Sanderson, S. D. et al. Innate immune induction and influenza protection elicited by a response-selective agonist of human C5a. PLoS One 7, e40303 (2012). 22792270

• Sample Type Confirmation:

  IFNB1 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: NP_002167.1
• Gene Symbol: IFNB1
• Predicted Species Reactivity: Human
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HepG2
  
  Western blot analysis of IFNB1 Antibody (N-term) in HepG2 cell lysates (35ug/lane). IFNB1 (arrow) was detected using the purified Pab.

  IFNB1 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Image 2: human hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human hepatocarcinoma with IFNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: HepG2
  
  Flow cytometric analysis of HepG2 cells using IFNB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.