BDNF Antibody - C-terminal region (OAAB05026)

Data Sheet
 
Product Number OAAB05026
Product Page www.avivasysbio.com/bdnf-antibody-c-terminal-region-oaab05026.html
Name BDNF Antibody - C-terminal region (OAAB05026)
Molecular Weight 27818 Da
Isotype Ig
NCBI Gene Id 627
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols ANON2, BULN2
Product Format Liquid. PBS with 0.09% (W/V) sodium azide.
Description of Target BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this protein is reduced in both Alzheimer's and Huntington disease patients. This protein may play a role in the regulation of stress response and in the biology of mood disorders.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-BDNF antibody - OAAB05026
Additional Information Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Cellular Location: Secreted.
Tissue Location: Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta
Application Info WB: 1:1000
IHC-P: 1:10~50
IF: 1:10~50
FC: 1:10~50
Other Applications Image 1 Data Flow cytometric analysis of CEM cells using BDNF Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunogen This BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF.
Intended Use For research use only and not for use in diagnostic or therapeutic procedures.
Uniprot ID P23560
Protein Name Brain-derived neurotrophic factor
Protein Accession # NP_001137277.1
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Gene Symbol BDNF
Predicted Species Reactivity Human
Application WB, IHC-P, FC, IF
Predicted Homology Based on Immunogen Sequence Bovine, Mouse, Pig, Rat
Image 1
CEM
Western blot analysis of BDNF Antibody (C-term) in CEM cell lysates (35ug/lane). BDNF (arrow) was detected using the purified Pab.
Image 2
human brain
Formalin-fixed and paraffin-embedded human brain reacted with BDNF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Image 3
CEM
Flow cytometric analysis of CEM cells using BDNF Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Image 4
Human Cerebellum and U-87 MG
All lanes : Anti-BDNF Antibody (C-term) at 1:1000 dilution Lane 1: human cerebellum lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Image 5
NCI-H460
Confocal immunofluorescent analysis of BDNF Antibody (C-term)(OAAB05026) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
 

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