SMAD4 Antibody - C-terminal region (OAAB04797)

Data Sheet
 
Product Number OAAB04797
Product Page www.avivasysbio.com/smad4-antibody-c-terminal-region-oaab04797.html
Name SMAD4 Antibody - C-terminal region (OAAB04797)
Molecular Weight 60439 Da
Isotype Rabbit Ig
NCBI Gene Id 4089
Host Rabbit
Clonality Polyclonal
Concentration 0.25 mg/ml
Alias Symbols JIP, DPC4, MADH4, MYHRS
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-SMAD4 antibody - OAAB04797
Application Info WB: 1:1000
IHC-P: 1:10~50
IF: 1:10~50
FC: 1:10~50
Other Applications Image 1 Data SMAD4 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunogen This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from the C-terminal region of human SMAD4.
Uniprot ID Q13485
Protein Name Mothers against decapentaplegic homolog 4
Protein Accession # NP_005350.1
Gene Symbol SMAD4
Predicted Species Reactivity Human
Application FC, IF, RIA
Predicted Homology Based on Immunogen Sequence Human
Image 1
293 cell
Western blot analysis of SMAD4 (arrow) using rabbit polyclonal SMAD4 Antibody (C-term) (Cat. #OAAB04797). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).
Image 2
Hela cell
Fluorescent confocal image of Hela cell stained with SMAD4 Antibody (C-term)(Cat#OAAB04797).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD4 primary antibody (1:25, 1 h at 37C). For secondary antibody, Alexa Fluor488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD4 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Image 3
human skeletal muscle
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Image 4
MCF-7 cell
SMAD4 Antibody (C-term) (Cat. #OAAB04797) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

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