| Product Number |
OAAB04732 |
| Product Page |
www.avivasysbio.com/kit-antibody-n-terminal-region-oaab04732.html |
| Name |
KIT Antibody - N-terminal region (OAAB04732) |
| Molecular Weight |
109864 Da |
| Isotype |
Rabbit Ig |
| NCBI Gene Id |
3815 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Alias Symbols |
PBT, SCFR, C-Kit, CD117, MASTC |
| Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-KIT antibody - OAAB04732 |
| Application Info |
WB: 1:1000
FC: 1:10~50
IHC-P: 1:10~50 |
| Other Applications Image 1 Data |
Flow cytometric analysis of NCI-H460 cells using KIT Antibody (N-term D121)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
| Immunogen |
This KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human KIT. |
| Uniprot ID |
Q99662 |
| Protein Name |
Mast/stem cell growth factor receptor Kit |
| Protein Accession # |
NP_000213.1 |
| Gene Symbol |
KIT |
| Predicted Species Reactivity |
Human, Mouse |
| Application |
FC, WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Human; Mouse |
| Image 1 | mouse testis
 | KIT Antibody (N-term D121) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow). |
| | Image 2 | human lung carcinoma
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with KIT antibody (N-term D121), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. |
| | Image 3 | NCI-H460
 | Flow cytometric analysis of NCI-H460 cells using KIT Antibody (N-term D121)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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