||ERBB2 Antibody (OAAB04692)
||Tyrosine kinase-type cell surface receptor HER2, ERBB2
||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|NCBI Gene Id
||Approximately 0.5mg/ml. Actual concentration varies with each lot.
|Reconstitution and Storage
||Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
||Printable datasheet for anti-ERBB2 antibody - OAAB04692
||Function: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
||Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1 Isoform 3: Cytoplasm. Nucleus.
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth
||This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1227-1255 amino acids from the C-terminal region of human ERBB2.
||Find tissues and cell lines supported by DNA array analysis to express ERBB2.
||Receptor tyrosine-protein kinase erbB-2
Suzuki, S. et al. Nucleobindin 2 in human breast carcinoma as a potent prognostic factor. Cancer Sci. 103, 136-43 (2012). WB, IHC, Human, Mouse, Rat 21988594
|Protein Accession #
||Find tissues and cell lines supported by RNA-seq analysis to express ERBB2.
||This antibody may replace item sc-08, HPA001383
|Predicted Species Reactivity
||Human, Mouse, Rat
|Predicted Homology Based on Immunogen Sequence
||Human; Mouse; Rat
|293 ||Western blot analysis of ERBB2(arrow) using rabbit polyclonal ERBB2 Antibody (Y1248) 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERBB2 gene (Lane 2) (Origene Technologies).|
|T47D||Western blot analysis of ERBB2 (arrow) in T47D cell lysates, either noninduced (Lane 1) or induced with HRG (Lane 2).(1:250)|
|human prostata carcinoma||Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with ErbB2-pY1248.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.|