ERBB2 Antibody (OAAB04692)

Data Sheet
Product Number OAAB04692
Product Page
Name ERBB2 Antibody (OAAB04692)
Gene Symbol ERBB2
Alias Symbols Tyrosine kinase-type cell surface receptor HER2, ERBB2
Molecular Weight 137909 Da
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Isotype Rabbit Ig
NCBI Gene Id 2064
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ERBB2 antibody - OAAB04692
Additional Information Function: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Additional Information Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1 Isoform 3: Cytoplasm. Nucleus.
Tissue Location:
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth
Application Info WB: 1:1000
IHC-P: 1:10~50
Immunogen This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1227-1255 amino acids from the C-terminal region of human ERBB2.
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ERBB2.
Swissprot Id P04626
Protein Name Receptor tyrosine-protein kinase erbB-2

Suzuki, S. et al. Nucleobindin 2 in human breast carcinoma as a potent prognostic factor. Cancer Sci. 103, 136-43 (2012). WB, IHC, Human, Mouse, Rat 21988594

Protein Accession # NP_001005862.1
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ERBB2.
Replacement Item This antibody may replace item sc-08, HPA001383
Predicted Species Reactivity Human, Mouse, Rat
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human; Mouse; Rat
Image 1
Western blot analysis of ERBB2(arrow) using rabbit polyclonal ERBB2 Antibody (Y1248) 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERBB2 gene (Lane 2) (Origene Technologies).
Image 2
Western blot analysis of ERBB2 (arrow) in T47D cell lysates, either noninduced (Lane 1) or induced with HRG (Lane 2).(1:250)
Image 3
human prostata carcinoma
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with ErbB2-pY1248.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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