SPRY4 Antibody (OAAB04476)

• Product Number: OAAB04476
• Name: SPRY4 Antibody (OAAB04476)
• Molecular Weight: 32541 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 81848
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: HH17
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1.
  Cellular Location: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side
  
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: SPRY4-Y75 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This SPRY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from human SPRY4.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Uniprot ID: Q9C004
• Protein Name: Protein sprouty homolog 4
• Sample Type Confirmation:

  SPRY4 is supported by BioGPS gene expression data to be expressed in K562

• Protein Accession #: NP_001120968.1
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: SPRY4
• Predicted Species Reactivity: Human
• Application: WB, FC, IHC-P
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: brain tissue
  
  SPRY4-Y75 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRY4-Y75 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 2: K562 cell
  
  Western blot analysis of Phospho-SPRY4-Y75.ctrl in K562 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab.
• Image 3: K562 cell
  
  SPRY4-Y75 Antibody flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.