CD163 Antibody - N-terminal region (OAAB04323)

• Product Number: OAAB04323
• Name: CD163 Antibody - N-terminal region (OAAB04323)
• Molecular Weight: 125451 Da
• Isotype: Ig
• NCBI Gene Id: 9332
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: M130, MM130, SCARI1
• Product Format: Liquid; PBS with 0.09% (W/V) sodium azide.
• Description of Target: CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
  
  Cellular Location Soluble CD163: Secreted.
  
  Tissue Location Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Immunogen: This CD163 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-274 amino acids from the N-terminal region of human CD163.
• Uniprot ID: Q86VB7
• Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• Sample Type Confirmation:

  CD163 is strongly supported by BioGPS gene expression data to be expressed in K562

• Protein Accession #: NP_004235.4
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: CD163
• Predicted Species Reactivity: Human
• Application: WB, IHC-P
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: K562
  
  Western blot analysis of CD163 antibody (N-term) in K562 cell lysates (35ug/lane). CD163 (arrow) was detected using the purified Pab.

  CD163 is strongly supported by BioGPS gene expression data to be expressed in K562

• Image 2: human hepatocarcinoma
  
  Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD163 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.