MFAP5 Antibody - C-terminal region (OAAB04309)

• Product Number: OAAB04309
• Name: MFAP5 Antibody - C-terminal region (OAAB04309)
• Molecular Weight: 20 kDa
• Isotype: Rabbit Ig
• NCBI Gene Id: 8076
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.6 mg/ml
• Alias Symbols: AAT9, MP25, MAGP2, MAGP-2, MFAP-5
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Description of Target: MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity. Component of the elastin-associated microfibrils.
  Cellular Location: Secreted, extracellular space, extracellular matrix.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: MFAP5 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This MFAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human MFAP5.
• Uniprot ID: Q13361
• Protein Name: Microfibrillar-associated protein 5
• Protein Accession #: NP_003471.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Tested Species Reactivity: Human
• Gene Symbol: MFAP5
• Application: WB, FC, IHC,
• Predicted Homology Based on Immunogen Sequence: Bovine, Mouse
• Image 1: K562
  
  Western blot analysis of MFAP5 antibody (C-term) in K562 cell lysates (35ug/lane). MFAP5 (arrow) was detected using the purified Pab.
• Image 2: MFAP5
  
  Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the MFAP5 gene.
• Image 3: Human Skeletal Muscle
  
  Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 4: K562
  
  MFAP5 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.