ITGAX Antibody - C-terminal region (OAAB04110)

• Product Number: OAAB04110
• Name: ITGAX Antibody - C-terminal region (OAAB04110)
• Molecular Weight: 128 kDa
• Isotype: Rabbit Ig
• NCBI Gene Id: 3687
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: CD11C, SLEB6
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
  IF: 1:10~50
• Other Applications Image 1 Data: Immunofluorescence analysis of ITGAX Antibody (C-term) with paraffin-embedded human hepatocarcinoma . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is P
• Other Applications Image 2 Data: Flow cytometric analysis of SK-Br-3 cells using ITGAX Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This ITGAX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1119-1145 amino acids from the C-terminal region of human ITGAX.
• Uniprot ID: P20702
• Protein Name: Integrin alpha-X
• Sample Type Confirmation:

  ITGAX is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

• Protein Accession #: NP_000878.2
• Gene Symbol: ITGAX
• Predicted Species Reactivity: Human
• Application: WB, IHC, FC, IF
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: MDA-MB231 cell
  
  Western blot analysis of ITGAX Antibody (C-term) (Cat. #OAAB04110) in MDA-MB231 cell line lysates (35ug/lane). ITGAX (arrow) was detected using the purified Pab.
• Image 2: paraffin-embedded human hepatocarcinoma . 0.05 mg/ml primary antibody was
  
  Immunofluorescence analysis of ITGAX Antibody (C-term) with paraffin-embedded human hepatocarcinoma. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
• Image 3: SK-Br-3 cell
  
  Flow cytometric analysis of SK-Br-3 cells using ITGAX Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 4: human hepatocarcinoma tissue
  
  Formalin-fixed and paraffin-embedded human hepatocarcinoma with ITGAX Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.