Product Number |
OAAB04005 |
Product Page |
www.avivasysbio.com/glul-antibody-n-terminal-region-oaab04005.html |
Name |
GLUL Antibody - N-terminal region (OAAB04005) |
Molecular Weight |
42 kDa |
Isotype |
Ig |
NCBI Gene Id |
2752 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Alias Symbols |
GS, GLNS, PIG43, PIG59 |
Product Format |
Liquid. PBS with 0.09% (W/V) sodium azide. |
Description of Target |
GLUL belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. |
Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-GLUL antibody - OAAB04005 |
Additional Information |
Function: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Cellular Location: Cytoplasm. Mitochondrion |
Application Info |
IHC-P: 1:25 WB: 1:2000 |
Immunogen |
This GLUL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-96 amino acids from the N-terminal region of human GLUL. |
Intended Use |
For research use only and not for use in diagnostic or therapeutic procedures. |
Uniprot ID |
P15104, P46410, P15105, Q4R7U3, P16580, P15103 |
Protein Name |
Glutamine synthetase |
Protein Accession # |
NP_001028216.1 |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Gene Symbol |
GLUL |
Predicted Species Reactivity |
Human, Mouse |
Application |
WB, IHC-P |
Predicted Homology Based on Immunogen Sequence |
Bovine, Chicken, Monkey, Pig |
Image 1 | mouse cerebellum
| Western blot analysis of GLUL Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). GLUL (arrow) was detected using the purified Pab. |
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Image 2 | Human Testis
| Formalin-fixed and paraffin-embedded human testis tissue reacted with GLUL Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Image 3 | Human Kidney
| OAAB04005 staining GLUL in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
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Image 4 | HeLa, HT-29, Jurkat, Mouse brain, Mouse cerebellum, Rat cerebellum
| Western blot analysis of lysates from Hela, HT-29, Jurkat cell line, mouse brain, mouse cerebellum, rat cerebellum tissue lysate(from left to right), using GLUL Antibody (N-term)(Cat. #OAAB04005). OAAB04005 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. |
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