FHL1 Antibody - C-terminal region (OAAB03956)

• Product Number: OAAB03956
• Name: FHL1 Antibody - C-terminal region (OAAB03956)
• Molecular Weight: 36263 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 2273
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: KYOT, SLIM, FCMSU, FHL-1, FHL1A, FHL1B, FLH1A, SLIM1, XMPMA, RBMX1A, RBMX1B, SLIM-1, SLIMMER
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of widr cells using FHL1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This FHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-240 amino acids from the C-terminal region of human FHL1.
• Uniprot ID: Q13642
• Protein Name: Four and a half LIM domains protein 1
• Protein Accession #: NP_001153171.1
• Gene Symbol: FHL1
• Predicted Species Reactivity: Human
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: NIH-3T3
  
  Western blot analysis of FHL1 Antibody (C-term) in NIH-3T3 cell lysates (35ug/lane). FHL1 (arrow) was detected using the purified Pab.
• Image 2: FHL1
  
  Western blot analysis of FHL1 (arrow) using rabbit polyclonal FHL1 Antibody (C-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the FHL1 gene.
• Image 3: Human Skeletal Muscle
  
  Formalin-fixed and paraffin-embedded human skeletal muscle reacted with FHL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 4: WiDr
  
  Flow cytometric analysis of widr cells using FHL1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.