HMGCS2 Antibody - C-terminal region (OAAB03887)

• Product Number: OAAB03887
• Name: HMGCS2 Antibody - C-terminal region (OAAB03887)
• Molecular Weight: 56635 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 3158
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: Hydroxymethylglutaryl-CoA synthase, mitochondrial, HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS2
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
• Additional Information: Cellular Location: Mitochondrion.
  Tissue Location: Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain. The relative expression of isoform 3 (at mRNA level) is highest in heart (70%) and skeletal muscle (60%)
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Immunogen: This HMGCS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 478-508 amino acids from the C-terminal region of human HMGCS2.
• Uniprot ID: P54868
• Protein Name: Hydroxymethylglutaryl-CoA synthase, mitochondrial
• Protein Accession #: NP_001159579.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: HMGCS2
• Predicted Species Reactivity: Human, Rat
• Application: WB, IHC, FC
• Predicted Homology Based on Immunogen Sequence: Human; Rat
• Image 1: human hepatocarcinoma
  
  HMGCS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HMGCS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 2: Human pancreas, liver, testis and Rat liver tissue
  
  Western blot analysis of lysates from human pancreas, liver, testis and rat liver tissue lysate (from left to right), using HMGCS2 Antibody (C-term)(Cat. #OAAB03887). OAAB03887 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
• Image 3: HepG2
  
  HMGCS2 Antibody (C-term) (Cat. #OAAB03887) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis