MLF1 Antibody - C-terminal region (OAAB03783)

• Product Number: OAAB03783
• Name: MLF1 Antibody - C-terminal region (OAAB03783)
• Molecular Weight: 30627 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 4291
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: WB: 1:1000
  FC: 1:10~50
  IHC-P: 1:10~50
• Other Applications Image 1 Data: MLF1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This MLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the C-terminal region of human MLF1.
• Uniprot ID: P58340
• Protein Name: Myeloid leukemia factor 1
• Sample Type Confirmation:

  MLF1 is supported by BioGPS gene expression data to be expressed in MCF7

• Protein Accession #: NP_001123628.1
• Gene Symbol: MLF1
• Predicted Species Reactivity: Human, Mouse
• Application: WB, FC, IHC
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: Mouse Heart
  
  Western blot analysis of MLF1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). MLF1 (arrow) was detected using the purified Pab.
• Image 2: MCF-7
  
  MLF1 Antibody (C-term) western blot analysis in MCF-7 cell lysates (35ug/lane).This demonstrates the MLF1 antibody detected the MLF1 protein (arrow).

  MLF1 is supported by BioGPS gene expression data to be expressed in MCF7

• Image 3: testis carcinoma
  
  MLF1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MLF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 4: CEM
  
  MLF1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.