PAX4 Antibody - middle region (OAAB03746)

Data Sheet
 
Product Number OAAB03746
Product Page www.avivasysbio.com/pax4-antibody-center-region-oaab03746.html
Name PAX4 Antibody - middle region (OAAB03746)
Molecular Weight 37833
Isotype Rabbit Ig
NCBI Gene Id 5078
Host Rabbit
Clonality Polyclonal
Concentration 2 mg/ml
Alias Symbols KPD, MODY9
Product Format Liquid PBS with 0.09% (W/V) sodium azide.
Description of Target Paired box protein Pax-4, paired box protein Pax-4 [Homo sapiens]
Reconstitution and Storage Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for PAX4 Antibody (Center) (OAAB03746)
Additional Information Cellular Location: Nucleus.
Application Info WB: 1:2000
IHC-P: 1:10~50
FC: 1:10~50
Other Applications Image 1 Data Flow cytometric analysis of widr cells using PAX4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunogen This PAX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human PAX4.
Uniprot ID https://www.uniprot.org/uniprot/?query=O43316+OR+P32115
Protein Name NP_006184.2, O43316, P32115
Protein Accession # https://www.ncbi.nlm.nih.gov/protein/NP_006184.2
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Tested Species Reactivity Human, Rat
Gene Symbol PAX4
Predicted Species Reactivity Human, Mouse, Rat
Application Flow cytometry, Immunohistochemistry-Paraffin, Western blot
Image 1
CEM
Western blot analysis of PAX4 antibody (Center) (RB20216) in CEM cell lysates (35ug/lane). PAX4 (arrow) was detected using the purified Pab.
Image 2
human brain
Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Image 3
WiDr
Flow cytometric analysis of WiDr cells using PAX4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

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