WNT10B Antibody - middle region (OAAB03615)

• Product Number: OAAB03615
• Name: WNT10B Antibody - middle region (OAAB03615)
• Molecular Weight: 43 kDa
• Isotype: Rabbit Ig
• NCBI Gene Id: 7480
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 2 mg/ml
• Alias Symbols: SHFM6, STHAG8, WNT-12
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Description of Target: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Secreted, extracellular space, extracellular matrix. Secreted.
  Tissue Location: Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
• Application Info: WB: 1:2000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This WNT10B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from the Central region of human WNT10B.
• Uniprot ID: O00744
• Protein Name: Protein Wnt-10b
• Protein Accession #: NP_003385.2
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Gene Symbol: WNT10B
• Predicted Species Reactivity: Human, Rat
• Application: WB, IHC-P, FC
• Image 1: human colon carcinoma
  
  WNT10B Antibody (Center) (Cat. #OAAB03615) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT10B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 2: MDA-231 cell
  
  Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 3: MDA-MB231 cell
  
  Western blot analysis of WNT10B Antibody (Center) (Cat. #OAAB03615) in MDA-MB231 cell line lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab.
• Image 4: mouse liver tissue
  
  Western blot analysis of WNT10B Antibody (Center) (Cat. #OAAB03615) in mouse liver tissue lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab.