CD40LG Antibody - C-terminal region (OAAB03549)

• Product Number: OAAB03549
• Name: CD40LG Antibody - C-terminal region (OAAB03549)
• Molecular Weight: 29274 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 959
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 2 mg/ml
• Alias Symbols: IGM, IMD3, TRAP, gp39, CD154, CD40L, HIGM1, T-BAM, TNFSF5, hCD40L
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T- cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity).
  Cellular Location: Cell membrane; Single-pass type II membrane protein Cell surface.
  Tissue Location: Specifically expressed on activated CD4+ T- lymphocytes.
  
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: TRAP Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-207 amino acids from the C-terminal region of human TRAP.
• Intended Use: For research use only.
• Uniprot ID: P29965
• Protein Name: CD40 ligand
• Sample Type Confirmation:

  CD40LG is strongly supported by BioGPS gene expression data to be expressed in K562

• Protein Accession #: NP_000065.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: CD40LG
• Predicted Species Reactivity: Human
• Application: WB, FC, IHC-P
• Image 1: K562
  
  Western blot analysis of TRAP Antibody (C-term) in K562 cell lysates (35ug/lane).TRAP (arrow) was detected using the purified Pab.

  CD40LG is strongly supported by BioGPS gene expression data to be expressed in K562

• Image 2: kidney carcinoma
  
  TRAP Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 3: K562
  
  TRAP Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.