ABCG1 Antibody - N-terminal region (OAAB03522)

• Product Number: OAAB03522
• Name: ABCG1 Antibody - N-terminal region (OAAB03522)
• Molecular Weight: 76 kDa
• Isotype: IgG
• NCBI Gene Id: 9619
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.38 mg/ml
• Alias Symbols: ABC8, WHITE1
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Description of Target: ABCG1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Mitochondrion. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes
  Tissue Location: Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
• Application Info: WB: 1:2000
  IF: 1:10~50
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: ABCG1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the N-terminal region of human ABCG1.
• Uniprot ID: P45844
• Protein Name: ATP-binding cassette sub-family G member 1
• Publications:

  Fu, Y. et al. ABCA12 Regulates ABCA1-Dependent Cholesterol Efflux from Macrophages and the Development of Atherosclerosis. Cell Metab. 18, 225-38 (2013). 23931754

• Protein Accession #: NP_004906.3
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: ABCG1
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IHC-P, FC, IF, ELISA
• Image 1: mouse spleen
  
  Western blot analysis of ABCG1 antibody (N-term) in mouse spleen tissue lysates (35ug/lane). ABCG1 (arrow) was detected using the purified Pab.
• Image 2: human lung carcinoma
  
  ABCG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
• Image 3: HepG2
  
  ABCG1 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Image 4: 293 cell
  
  Confocal immunofluorescent analysis of ABCG1 Antibody (N-term)(Cat#OAAB03522) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).