ENO1 Antibody - middle region (OAAB03519)

Data Sheet
 
Product Number OAAB03519
Product Page www.avivasysbio.com/eno1-antibody-center-region-oaab03519.html
Name ENO1 Antibody - middle region (OAAB03519)
Molecular Weight 47 kDa
Isotype Rabbit Ig
NCBI Gene Id 2023
Host Rabbit
Clonality Polyclonal
Concentration 2 mg/ml
Alias Symbols NNE, PPH, MPB1, ENO1L1, HEL-S-17
Product Format Liquid. PBS with 0.09% (W/V) sodium azide.
Description of Target ENO1 is one of three enolase isoenzymes found in mammals; it is alpha-enolase, a homodimeric soluble enzyme, and also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ENO1 antibody - OAAB03519
Additional Information Function: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Cellular Location: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
Tissue Location: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Application Info WB: 1:1000
IHC-P: 1:10~50
FC: 1:10~50
IF: 1:10~50
Immunogen This ENO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the Central region of human ENO1.
Intended Use For research use only and not for use in diagnostic or therapeutic procedures.
Uniprot ID P06733
Protein Name Alpha-enolase
Protein Accession # NP_001188412.1
Purification This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Gene Symbol ENO1
Predicted Species Reactivity Human, Mouse
Application WB, IHC-P, IF, FC
Predicted Homology Based on Immunogen Sequence Cow, Chicken, Monkey, Rat, Xenopus
Image 1
Jurkat, MCF-7, U251, mouse NIH/3T3 cell, mouse brain tissue
ENO1 Antibody (Center) (Cat. #OAAB03519) western blot analysis in Jurkat, MCF-7, U251, mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).
Image 2
Hela cell
ENO1 Antibody (Center) (Cat. #OAAB03519) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Image 3
C2C12 cell
Image 4
human lymph
Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
 

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